Journal: PLoS Pathogens
Article Title: In vitro and in vivo characterization of a recombinant rhesus cytomegalovirus containing a complete genome
doi: 10.1371/journal.ppat.1008666
Figure Lengend Snippet: A phylogenetic tree for FL-RhCMV and rodent and primate CMVs was constructed based on full genome alignments using the Geneious Prime Tree Builder application. Sequences previously published include RhCMV 180.92 as well as the RhCMV isolates 19262, 19936 and 24514 and the Cynomolgus CMV isolates 31906, 31907, 31908 and 31909 . We also included the published sequences for the CyCMV strains Ottawa and Mauritius , the simian (African green monkey) CMV isolates Colburn , GR2715 and stealth virus 1 as well as the BaCMV strains OCOM4-37 and OCOM4-52 and the DrCMV strain OCOM6-2 . For comparison we included the HCMV TR3 strain , the chimpanzee CMV strain Heberling and the only two complete genome sequences of new world NHP CMVs, Aotine betaherpesvirus 1 (AoHV-1) strain S34E and Saimiriine betaherpesvirus 4 (SaHV-4) strain SqSHV . New genome sequences included in this alignment are as follows: the two RhCMV isolates 34844 and KF03, the CyCMV isolate 31709, the Japanese macaque CMV JaCMV 24655 and the two baboon CMVs BaCMV 31282 and 34826. These CMVs were isolated from fibroblast co-cultures of urine samples obtained from NHP housed either at the Oregon National Primate Research Center (ONPRC) or the Tulane National Primate Research Center (TNPRC). Also included in the alignment are the genomic sequences of the previously published RhCMV isolates UCD52 and UCD59 that originated at the UC Davis National Primate Research Center [ , ]. The rodent CMVs include the rat CMV (RCMV) isolates Maastricht , England and Berlin , the guinea pig CMV (GPCMV) isolate 22122 and the murine CMV (MCMV) strain Smith , which was used as an outgroup.
Article Snippet: Genome annotations were fine-tuned utilizing these and other NHP CMV sequences that had been previously submitted to GenBank, either by us: CyCMV 31906 (KX689263), CyCMV 31907 (KX689264), CyCMV 31908 (KX689265), CyCMV 31909 (KX689266), RhCMV 180.92 (DQ120516, AAZ80589), RhCMV 19262 (KX689267), RhCMV 19936 (KX689268) and RhCMV 24514 (KX689269) or by others: CyCMV Ottawa (JN227533, AEQ32165), CyCMV Mauritius (KP796148, AKT72642), SCMV Colburn (FJ483969, AEV80601), SCMV GR2715 (FJ483968, AEV80365), SCMV Stealth virus 1 strain ATCC VR-2343 (U27469, U27238, U27770, U27627, U27883, U27471), BaCMV OCOM4-37 (AC090446), BaCMV OCOM4-52 (KR351281, AKG51610.1), DrCMV OCOM6-2 (KR297253, AKI29779).
Techniques: Construct, Virus, Comparison, Isolation, Genomic Sequencing
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![A phylogenetic tree for FL-RhCMV and rodent and primate CMVs was constructed based on full genome alignments using the Geneious Prime Tree Builder application. Sequences previously published include RhCMV 180.92 as well as the RhCMV isolates 19262, 19936 and 24514 and the Cynomolgus CMV isolates 31906, 31907, 31908 and 31909 . We also included the published sequences for the CyCMV strains Ottawa and Mauritius , the simian (African green monkey) CMV isolates Colburn , <t>GR2715</t> and stealth virus 1 as well as the BaCMV strains OCOM4-37 and OCOM4-52 and the DrCMV strain OCOM6-2 . For comparison we included the HCMV TR3 strain , the chimpanzee CMV strain Heberling and the only two complete genome sequences of new world NHP CMVs, Aotine betaherpesvirus 1 (AoHV-1) strain S34E and Saimiriine betaherpesvirus 4 (SaHV-4) strain SqSHV . New genome sequences included in this alignment are as follows: the two RhCMV isolates 34844 and KF03, the CyCMV isolate 31709, the Japanese macaque CMV JaCMV 24655 and the two baboon CMVs BaCMV 31282 and 34826. These CMVs were isolated from fibroblast co-cultures of urine samples obtained from NHP housed either at the Oregon National Primate Research Center (ONPRC) or the Tulane National Primate Research Center (TNPRC). Also included in the alignment are the genomic sequences of the previously published RhCMV isolates UCD52 and UCD59 that originated at the UC Davis National Primate Research Center [ , ]. The rodent CMVs include the rat CMV (RCMV) isolates Maastricht , England and Berlin , the guinea pig CMV (GPCMV) isolate 22122 and the murine CMV (MCMV) strain Smith , which was used as an outgroup.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_3282/pmc07723282/pmc07723282__ppat.1008666.g002.jpg)